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SCI 18 September 2024 A mass cytometry method pairing T cell receptor and differentiation state analysis (Nat Immunol; IF:27.7) Garcia Castillo J, DeBarge R, Mende A, Tenvooren I, Marquez DM, Straub A, Busch DH, Spitzer MH, DuPage M: A mass cytometry method pairing T cell receptor and differentiation state analysis. Nat Immunol 2024, 25(9):1754-1763. Correspondence to: matthew.spitzer@ucsf.edu; dupage@berkeley.edu T cell antigen receptor (TCR) recognition followed by clonal expansion is a fundamental feature of adaptive immune responses. Here, we present a mass cytometric (CyTOF) approach to track T cell responses by combining antibodies for specific TCR Vα and Vβ chains with antibodies against T cell activation and differentiation proteins in mice. This strategy identifies expansions of CD8+ and CD4+ T cells expressing specific Vβ and Vα chains with varying differentiation states in response to Listeria monocytogenes, tumors and respiratory influenza infection. Expanded T cell pop
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