生物信息学近期高水平文献速递（1.22)

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1.无引物偏好检索100万高质量，全长微生物16S和18S rRNA基因序列 Retrieval of a million high-quality, full-length microbial 16S and 18S rRNA gene sequences without primer bias Nature Biotechnology Abstract Small subunit ribosomal RNA (SSU rRNA) genes, 16S in bacteria and 18S in eukaryotes, have been the standard phylogenetic markers used to characterize microbial diversity and evolution for decades. However, the reference databases of full-length SSU rRNA gene sequences are skewed to well-studied ecosystems and subject to primer bias and chimerism, which results in an incomplete view of the diversity present in a sample. We combine poly(A)-tailing and reverse transcription of SSU rRNA molecules with synthetic long-read sequencing to generate high-quality, full-length SSU rRNA sequences, without primer bias, at high throughput. We apply our approach to sam ………………………………