David Baker｜变构蛋白设计

文章预览

David Baker｜变构蛋白设计 David Baker的最新预印版论文，对变构蛋白进行设计。大家和一作Adam Broerman一起来看看这篇论文吧。 Adam Broerman Protein interaction kinetics often affect the dynamic behavior of biological systems. While high affinity interactions are useful, they usually exchange slowly due to their low off-rates. We developed a general strategy for inducing rapid exchange of high affinity interactions. 蛋白质相互作用动力学通常影响生物系统的动态行为。虽然高亲和力相互作用很有用，但由于它们的解离速率低，它们通常交换得很慢。我们开发了一种通用策略来诱导高亲和力相互作用的快速交换。 Adam Broerman We designed an effector-responsive conformational switch which can be attached to almost any binder to sterically modulate binding its partner. Binding the effector induces a strained intermediate state from which partner dissociation is accelerated by up to thousa ………………………………