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三代测序探索者每周文献精选 （09.23-09.29 ） 01 Strainy: phasing and assembly of strain haplotypes from long-read metagenome sequencing 杂志：Nature methods（IF=36.1） 发表时间：2024.09.26 第一作者：Ekaterina Kazantseva（ITMO University） 通讯作者：Mihai Pop（University of Maryland） 原文链接：https://doi.org/10.1101/2023.01.31.526521 解度链接：https://mp.weixin.qq.com/s/RCieMiCfNqCcH-s8Jty3bw Figure1.Long-read metagenome phasing algorithm. 亮点： Here we present Strainy, an algorithm for strain-level metagenome assembly and phasing from Nanopore and PacBio reads. Strainy takes a de novo metagenomic assembly as input and identifies strain variants, which are then phased and assembled into contiguous haplotypes. Using simulated and mock Nanopore and PacBio metagenome data, we show that Strainy assembles accurate and complete strain haplotypes, outperforming current Nanopore-based methods and comparable with PacBio-based algorithms
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