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三代测序探索者每周文献精选 （10.14-10.20 ） 01 Nanopore ultra-long sequencing and adaptive sampling spur plant complete telomere-to-telomere genome assembly 杂志：Molecular Plant（IF=17.1） 发表时间：2024.10.16 第一作者：Dongdong Lu（Peking University Institute of Advanced Agricultural Sciences） 通讯作者：Bosheng Li（Peking University Institute of Advanced Agricultural Sciences） 原文链接：https://www.sciencedirect.com/science/article/pii/S1674205224003307 解度链接： https://mp.weixin.qq.com/s/0AUQ26UeYARSYgz6KJvW3g Figure1. Advancements in ONT sequencing improve read lengths 亮点： In this study, we refined the technique for extracting plant DNA and preparing libraries for Nanopore sequencing . Our method focuses on isolating highly purified nuclei to obtain ultra-long DNA fragments. By minimizing the DNA input and limiting the presence of short fragments in the library, we significantly extended the sequencing read lengths. 02 Th
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