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SCI 3 August 2024 In vivo single-cell CRISPR uncovers distinct TNF programmes in tumour evolution (Nature; IF:50.5) Renz PF, Ghoshdastider U, Baghai Sain S et al. In vivo single-cell CRISPR uncovers distinct TNF programmes in tumour evolution. Nature, 2024 Correspondence to: andreas.moor@bsse.ethz.ch; ataman.sendoel@uzh.ch The tumour evolution model posits that malignant transformation is preceded by randomly distributed driver mutations in cancer genes, which cause clonal expansions in phenotypically normal tissues. Although clonal expansions can remodel entire tissues, the mechanisms that result in only a small number of clones transforming into malignant tumours remain unknown. Here we develop an in vivo single-cell CRISPR strategy to systematically investigate tissue-wide clonal dynamics of the 150 most frequently mutated squamous cell carcinoma genes. We couple ultrasound-guided in utero lentiviral microinjections, single-cell RNA sequencing and guide capture to longitudinally mo
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