文章预览
前期文章 一个超简单的转录组项目全过程--iMac+RNA-Seq(一) 一个超简单的转录组项目全过程--iMac+RNA-Seq(二)QC 一个超简单的转录组项目全过程--iMac+RNA-Seq(三)Alignment 比对 4 featureCounts 比对结束后需要进行计数咯! 老规矩,先看说明书。 featureCounts - h ## 好像发现了什么不得了的 $featureCounts - h featureCounts : invalid option - h Version 1.6.2 Usage: featureCounts [options] -a -o input file1 [input file2] ... -T number of threads -p If specified, fragments (or templates) will be counted instead of reads. This option is only applicable for paired-end reads . -t Specify feature type in GTF annotation. 'exon' by default . Features used for read counting will be extracted from annotation using the provided value. -g Specify attribute type in GTF annotation. ' gene_id' by default . M
………………………………