JAR丨中科院一区丨脑出血后，受损的小胶质细胞糖酵解通过HK2依赖性线粒体功能障碍促进炎症反应

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Chestnut Studying       摘要  Introduction: Intracerebral haemorrhage (ICH) is a devastating disease that leads to severe neurological deficits. Microglia are the first line of defence in the brain and play a crucial role in neurological recovery after ICH, whose activities are primarily driven by glucose metabolism. However, little is known regarding the status of glucose metabolism in microglia and its interactions with inflammatory responses after ICH. Objectives: This study investigated microglial glycolysis and its mechanistic effects on microglial inflammation after ICH. Methods: We explored the status of glucose metabolism in the ipsilateral region and in fluorescence-activated-cell-sorting-isolated (FACS-isolated) microglia via 2-deoxy-[18F]fluoro-D-glucose positron emission tomography (FDG-PET) analyses and gamma emission, respectively. Energy-related targeted metabolomics, along with 13C-glucose isotope tracing, was utilised to analyse glycolytic products in microglia. ………………………………