这种作者只提供了每组多个重复样本合并后数据的怎么办

文章预览

我们生信入门马拉松授课群里有个学员分析一个GEO数据时，发现这个数据很特别，他的提问： GSE65031这种作者只提供了合并数据的怎么办？ 来看下是怎么个合并情况吧！ 数据背景 GSE65031 这个数据为小鼠肺组织样本， 总共有4个分组为三个时间节点 + 一个对照，每个分组有5个样本，但是 测序的时候呢同一个组别中的这五个样本的 RNA 进行了 pool ，然后再测序 。 具体描述如下： Balb/cJ mice (5 per group) were intranasally challenged with 8-oxoguanine (1 µM, 60 µl) for three times at days 0, 2 and 4. Control group mice were intranasally challenged with saline (60 µl). At 30, 60 and 120 min after the third challenge (day 4), mice were sacrificed and lungs were processed for RNA extraction. RNAs from individual mice within the same group were pooled and subjected to deep-sequencing analysis in duplicate using NSG on an Illumina HiSeq 1000 sequencing system. After al ………………………………