【实验步骤】细胞荧光成像

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用激光共聚焦显微镜(CLSM)(徕卡SP8)获取细胞荧光图像，用Image J软件测定相对荧光强度。将4T1细胞以8 × 104个/孔的密度接种于2 mL DMEM培养基中，孵育24 h。 实验分为对照组、E (50 V)组、Ru800 (200 μg/mL)组、Ru900 (200 μg/mL)组、Ru1000 (200 μg/mL)组、Ru1000 + E (200 μg/mL, 50 V)组。在活细胞和死细胞分析中，在不同条件下处理后，用Calcein-AM和PI染色，并在CLSM上观察活细胞和死细胞的分化情况。 e Laser confocal images and (f) green/red fluorescence (FL) ratio of 4T1 cells stained with Calcein-AM (live cells: green) and propidium iodide (dead cells: red) after various treatments. g Laser confocal images of intracellular ROS level stained with DCFH-DA in 4T1 cells and h corresponding quantitative data of intracellular ROS levels. Data are presented as mean values ± SD. i JC-1 stained laser confocal image and (j) green/red FL ratios of 4T1 cells.  参考文献： Lattice expans ………………………………